Thứ Ba, 11 tháng 2, 2014

Tài liệu Antiviral Vaccines pptx


Socioeconomic and political
problems prevent many developing
nations from receiving vaccines

Inability to develop effective vaccines
for some pathogens

Vaccine-associated risks discourage
investment in developing new
vaccines
Vaccination Problems

3 general types of vaccines

Attenuated (live)

Killed (inactivated)

Toxoid
Vaccine Types

Also called modified live vaccines

Uses pathogens that are living but
have reduced virulence so they don’t
cause disease

Attenuation is the process of reducing
virulence

Viruses often attenuated by raising them in tissue
culture cells for which they aren’t adapted until
they lose the ability to produce disease

Bacteria can be made avirulent by culturing
under unusual conditions or through genetic
manipulation
Attenuated Vaccines

Can result in mild infections but no
disease

Contain replicating microbes that can
stimulate a strong immune response due
to the large number of antigen molecules

Viral vaccines trigger a cell-mediated
immune response dominated by T
H
1 and
cytotoxic T cells

Vaccinated individuals can infect those
around them, providing herd immunity
Attenuated Vaccines

Attenuated microbes may retain enough
virulence to cause disease, especially in
immunosuppressed individuals

Pregnant women should not receive live
vaccines due to the risk of the modified
pathogen crossing the placenta

Modified viruses may occasionally revert
to wild type or mutate to a virulent form
Problems with Attenuated
Vaccines

Can be either whole agent vaccines
produced with deactivated but whole
microbes, or subunit vaccines produced
with antigenic fragments of microbes

Both types are safer than live vaccines
since they cannot replicate or mutate to a
virulent form

When microbes are killed must not alter
the antigens responsible for stimulating
protective immunity
Inactivated Vaccines
Inactivated Vaccines (cont.)

Formaldehyde is commonly used to
inactivate microbes by cross-linking their
proteins and nucleic acids

Recognized as exogenous antigens and
stimulate a T
H
2 response that promotes
antibody-mediated immunity

Do not stimulate herd immunity

Whole agent vaccines may
stimulate a inflammatory response
due to nonantigenic portions of the
microbe

Antigenically weak since the
microbes don’t reproduce and don’t
provide many antigenic molecules
to stimulate the immune response
Problems with Inactivated
Vaccines

Administration in high or multiple
doses, or the incorporation of an
adjuvant, can make the vaccine more
effective

Adjuvants are substances that increase
the antigenicity of the vaccine

Adjuvants may also stimulate local
inflammation

High and multiple vaccine doses may
produce allergic reactions
Problems with Inactivated
Vaccines

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